dos.step 1. Test Collection

Due to the fact experimental product because of it studies, 72 staff member bees ( Apis cerana cerana ) was in fact obtained from 9 additional geographic hill parts when you look at the Main Asia (Table step 1 and Profile 1). All the honeybee trials was basically stored in a beneficial ?80 °C fridge for further DNA removal and you will sequencing.

2.2. DNA Extraction

Per bee colony, you to definitely staff bee was at random chosen, and overall genomic DNA are extracted from its thorax tissues using brand new Universal Genome DNA System (Cat.Zero.CW0553, ComWin Biotech, Beijing, China) with regards to the maker’s process. Leia mais